Whole-Cell-Based Photosynthetic Biohybrid Systems for Energy and Environmental Applications.
Li Luxuan,Xu Zhijun,Huang Xin
ChemPlusChem
With the increasing awareness of sustainable development, energy and environment are becoming two of the most important issues of concern to the world today. Whole-cell-based photosynthetic biohybrid systems (PBSs), an emerging interdisciplinary field, are considered as attractive biosynthetic platforms with great prospects in energy and environment, combining the superiorities of semiconductor materials with high energy conversion efficiency and living cells with distinguished biosynthetic capacity. This review presents a systematic discussion on the synthesis strategies of whole-cell-based PBSs that demonstrate a promising potential for applications in sustainable solar-to-chemical conversion, including light-facilitated carbon dioxide reduction and biohydrogen production. In the end, the explicit perspectives on the challenges and future directions in this burgeoning field are discussed.
10.1002/cplu.202100171
Liquid-Liquid Phase Separation-Mediated Photocatalytic Subcellular Hybrid System for Highly Efficient Hydrogen Production.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Plant chloroplasts have a highly compartmentalized interior, essential for executing photocatalytic functions. However, the construction of a photocatalytic reaction compartment similar to chloroplasts in inorganic-biological hybrid systems (IBS) has not been reported. Drawing inspiration from the compartmentalized chloroplast and the phenomenon of liquid-liquid phase separation, herein, a new strategy is first developed for constructing a photocatalytic subcellular hybrid system through liquid-liquid phase separation technology in living cells. Photosensitizers and in vivo expressed hydrogenases are designed to coassemble within the cell to create subcellular compartments for synergetic photocatalysis. This compartmentalization facilitates efficient electron transfer and light energy utilization, resulting in highly effective H production. The subcellular compartments hybrid system (HM/IBSCS) exhibits a nearly 87-fold increase in H production compared to the bare bacteria/hybrid system. Furthermore, the intracellular compartments of the photocatalytic reactor enhance the system's stability obviously, with the bacteria maintaining approximately 81% of their H production activity even after undergoing five cycles of photocatalytic hydrogen production. The research brings forward visionary prospects for the field of semi-artificial photosynthesis, offering new possibilities for advancements in areas such as renewable energy, biomanufacturing, and genetic engineering.
10.1002/advs.202400097
Retraction.
International journal of cancer
Iwase M, Watanabe H, Kondo G, Ohashi M, Nagumo M. Enhanced susceptibility of oral squamous cell carcinoma cell lines to FAS-mediated apoptosis by cisplatin and 5-fluorouracil. Int J Cancer. 2003 Sep 10;106(4):619-25. doi:10.1002/ijc.11239 The above article, published online on May 30, 2003, in Wiley Online Library (https://onlinelibrary.wiley.com/doi/10.1002/ijc.11239), has been retracted by agreement between the journal Editor-in-Chief (Prof. Christoph Plass), the authors, and Wiley Periodicals LLC. An Expression of Concern (https://onlinelibrary.wiley.com/doi/10.1002/ijc.33825) was published during an earlier stage of this investigation. The retraction has been agreed following internal analyses and an investigation by the author's institution. The investigation concluded that data fabrication had taken place during compilation of the figures and that the manuscript was submitted without approval of the co-authors. As a result, the overall conclusions of this manuscript are considered invalid.
10.1002/ijc.34482
MR-1 respires CdSe quantum dots for photocatalytic hydrogen evolution.
Proceedings of the National Academy of Sciences of the United States of America
Living bio-nano systems for artificial photosynthesis are of growing interest. Typically, these systems use photoinduced charge transfer to provide electrons for microbial metabolic processes, yielding a biosynthetic solar fuel. Here, we demonstrate an entirely different approach to constructing a living bio-nano system, in which electrogenic bacteria respire semiconductor nanoparticles to support nanoparticle photocatalysis. Semiconductor nanocrystals are highly active and robust photocatalysts for hydrogen (H) evolution, but their use is hindered by the oxidative side of the reaction. In this system, MR-1 provides electrons to a CdSe nanocrystalline photocatalyst, enabling visible light-driven H production. Unlike microbial electrolysis cells, this system requires no external potential. Illuminating this system at 530 nm yields continuous H generation for 168 h, which can be lengthened further by replenishing bacterial nutrients.
10.1073/pnas.2206975120
Reversing Electron Transfer Chain for Light-Driven Hydrogen Production in Biotic-Abiotic Hybrid Systems.
Journal of the American Chemical Society
The biotic-abiotic photosynthetic system integrating inorganic light absorbers with whole-cell biocatalysts innovates the way for sustainable solar-driven chemical transformation. Fundamentally, the electron transfer at the biotic-abiotic interface, which may induce biological response to photoexcited electron stimuli, plays an essential role in solar energy conversion. Herein, we selected an electro-active bacterium MR-1 as a model, which constitutes a hybrid photosynthetic system with a self-assembled CdS semiconductor, to demonstrate unique biotic-abiotic interfacial behavior. The photoexcited electrons from CdS nanoparticles can reverse the extracellular electron transfer (EET) chain within MR-1, realizing the activation of a bacterial catalytic network with light illumination. As compared with bare MR-1, a significant upregulation of hydrogen yield (711-fold), ATP, and reducing equivalent (NADH/NAD) was achieved in the MR-1-CdS under visible light. This work sheds light on the fundamental mechanism and provides design guidelines for biotic-abiotic photosynthetic systems.
10.1021/jacs.2c00934
Retraction.
Journal of cellular physiology
Retraction: "Effects of IGFBP3 gene silencing mediated inhibition of ERK/MAPK signaling pathway on proliferation, apoptosis, autophagy, and cell senescence in rats nucleus pulposus cells," by Gang Chen, Xiaopeng Zhou, and Zhengkuan Xu, J Cell Physiol. 2019; 9308-9315: The above article, published online on 28 October 2018 in Wiley Online Library (https://onlinelibrary.wiley.com/doi/full/10.1002/jcp.27613), and a corrsponding Erratum, published online on 6 May 2019 (https://doi.org/10.1002/jcp.28675) have been retracted by agreement between the authors, the journal's Editor in Chief, Prof. Dr. Gregg Fields, and Wiley Periodicals LLC. The retraction has been agreed after the authors stated that the experimental data in the article could not be verified. The investigation additionally revealed duplication in several image elements. Thus, the editors consider the conclusions of this article to be invalid. The authors were not available for a final confirmation of the retraction.
10.1002/jcp.30894
Quantitative, time-resolved proteomic analysis by combining bioorthogonal noncanonical amino acid tagging and pulsed stable isotope labeling by amino acids in cell culture.
Bagert John D,Xie Yushu J,Sweredoski Michael J,Qi Yutao,Hess Sonja,Schuman Erin M,Tirrell David A
Molecular & cellular proteomics : MCP
An approach to proteomic analysis that combines bioorthogonal noncanonical amino acid tagging (BONCAT) and pulsed stable isotope labeling with amino acids in cell culture (pSILAC) provides accurate quantitative information about rates of cellular protein synthesis on time scales of minutes. The method is capable of quantifying 1400 proteins produced by HeLa cells during a 30 min interval, a time scale that is inaccessible to isotope labeling techniques alone. Potential artifacts in protein quantification can be reduced to insignificant levels by limiting the extent of noncanonical amino acid tagging. We find no evidence for artifacts in protein identification in experiments that combine the BONCAT and pSILAC methods.
10.1074/mcp.M113.031914
Enzyme-Polymer-Conjugate-Based Pickering Emulsions for Cell-Free Expression and Cascade Biotransformation.
Angewandte Chemie (International ed. in English)
In this study, we addressed the limitations of conventional enzyme-polymer-conjugate-based Pickering emulsions for interfacial biocatalysis, which traditionally suffer from nonspecific and uncontrollable conjugation positions that can impede catalytic performance. By introducing a non-canonical amino acid (ncAA) at a specific site on target enzymes, we enabled precise polymer-enzyme conjugation. These engineered conjugates then acted as biocatalytically active emulsifiers to stabilize Pickering emulsions, while encapsulating a cell-free protein synthesis (CFPS) system in the aqueous phase for targeted enzyme expression. The resulting cascade reaction system leveraged enzymes expressed in the aqueous phase and on the emulsion interface for optimized chemical biosynthesis. The use of the cell-free system eliminated the need for intact whole cells or purified enzymes, representing a significant advancement in biocatalysis. Remarkably, the integration of Pickering emulsion, precise enzyme-polymer conjugation, and CFPS resulted in a fivefold enhancement in catalytic performance as compared to traditional single-phase reactions. Therefore, our approach harnesses the combined strengths of advanced biochemical engineering techniques, offering an efficient and practical solution for the synthesis of value-added chemicals in various biocatalysis and biotransformation applications.
10.1002/anie.202312906
Microglia-Mediated Neuroinflammation: A Potential Target for the Treatment of Cardiovascular Diseases.
Journal of inflammation research
Microglia are tissue-resident macrophages of the central nervous system (CNS). In the CNS, microglia play an important role in the monitoring and intervention of synaptic and neuron-level activities. Interventions targeting microglia have been shown to improve the prognosis of various neurological diseases. Recently, studies have observed the activation of microglia in different cardiovascular diseases. In addition, different approaches that regulate the activity of microglia have been shown to modulate the incidence and progression of cardiovascular diseases. The change in autonomic nervous system activity after neuroinflammation may be a potential intermediate link between microglia and cardiovascular diseases. Here, in this review, we will discuss recent updates on the regulatory role of microglia in hypertension, myocardial infarction and ischemia/reperfusion injury. We propose that microglia serve as neuroimmune modulators and potential targets for cardiovascular diseases.
10.2147/JIR.S350109
Thailandepsin a.
Acta crystallographica. Section E, Structure reports online
THAILANDEPSIN A [SYSTEMATIC NAME: (E)-(1S,5S,6R,9S,20R)-6-[(2S)-butan-2-yl]-5-hy-droxy-20-[2-(meth-yl-sulfan-yl)eth-yl]-2-oxa-11,12-dithia-7,19,22-triaza-bicyclo-[7.7.6]docosa-15-ene-3,8,18,21-tetra-one], C(23)H(37)N(3)O(6)S(3), is a newly reported [Wang et al. (2011). J. Nat. Prod. doi:10.1021/np200324x] bicyclic depsipeptide that has potent histone deacetyl-ase inhibitory activity and broad-spectrum anti-proliferative activity. The absolute configuration of thailandepsin A has been determined from the anomalous dispersion and the stereochemistry of all chiral C atoms. Intra-molecular N-H⋯O and N-H⋯S hydrogen bonds occur. Inter-molecular N-H⋯O and O-H⋯O hydrogen bonds are observed in the crystal structure.
10.1107/S1600536811041390
Enhanced Light-Driven Hydrogen Production by Self-Photosensitized Biohybrid Systems.
Martins Mónica,Toste Catarina,Pereira Inês A C
Angewandte Chemie (International ed. in English)
Storage of solar energy as hydrogen provides a platform towards decarbonizing our economy. One emerging strategy for the production of solar fuels is to use photocatalytic biohybrid systems that combine the high catalytic activity of non-photosynthetic microorganisms with the high light-harvesting efficiency of metal semiconductor nanoparticles. However, few such systems have been tested for H production. We investigated light-driven H production by three novel organisms, Desulfovibrio desulfuricans, Citrobacter freundii, and Shewanella oneidensis, self-photosensitized with cadmium sulfide nanoparticles, and compared their performance to Escherichia coli. All biohybrid systems produced H from light, with D. desulfuricans-CdS demonstrating the best activity overall and outperforming the other microbial systems even in the absence of a mediator. With this system, H was continuously produced for more than 10 days with a specific rate of 36 μmol g h . High apparent quantum yields of 23 % and 4 % were obtained, with and without methyl viologen, respectively, exceeding values previously reported.
10.1002/anie.202016960