Investigation of the differences between the "Cold" and "Hot" nature of Coptis chinensis Franch and its processed materials based on animal's temperature tropism. Zhou CanPing,Wang JiaBo,Zhang XueRu,Zhao YanLing,Xia XinHua,Zhao HaiPing,Ren YongShen,Xiao XiaoHe Science in China. Series C, Life sciences The description and differentiation of the so-called "Cold" and "Hot" natures, the primary "Drug Naure" of Chinese medicine, is the focus of theoretical research. In this study, the divergency between the "Cold" and the "Hot" natures was investigated through examining the temperature tropism of mice affected by Coptis chinensis Franch and its processed materials by using a cold/hot plate differentiating technology. After exposure to C. chinensis Franch, the macroscopic behavioral index of the remaining rate (RR) on a warm pad (40 degrees C) significantly increased (P<0.05), suggesting the enhancement of Hot tropism. The internal indexes of adenosine triphosphatase (ATPase) activity and oxygen consuming volume decreased significantly (P<0.05), suggesting the decapability of energy metabolism. This external behavior of Hot tropism might reflect the internal Cold nature of C. chinensis Franch. However, the processed materials of C. chinensis Franch exhibited a different Cold nature in temperature tropism compared with crude C. chinensis Franch (CC): the Cold nature of bile-processed C. chinensis Franch (BC) enhanced while the ginger-processed C. chinensis Franch (GC) changed inversely. The changing sequence was consistent with the theoretical prognostication. It is indicated that the external Cold & Hot natures of Chinese medicine may possibly reflect in an ethological way for the changes of animal's temperature tropism which might be internally regulated by the body's energy metabolism. 10.1007/s11427-009-0146-9

Study on the complex prescription compatibility law of the cold and hot nature of Mahuang Decoction and its categorized formulae based on the cold-hot pad differentiating assay. Jia Lei,Zhao Yan-Ling,Wang Jia-Bo,Zou Wen-Jun,Li Rui-Sheng,Yang Hong-Bo,Cheng Dan-Hong,Xiao Xiao-He Chinese journal of integrative medicine OBJECTIVE:To explore the complex prescription compatibility law of the cold and hot nature of Mahuang Decoction (, MHD) and Maxing Shigan Decoction (, MXSGD), both categorized both categorized MHD) and Maxing Shigan Decoction (both categorized MXSGD), both categorized formulas but with different hot/cold natures. METHODS:Oxygen consumption of mice was determined among three groups: MHD, MXSGD and the control; a cold-hot pad differentiating assay was used to observe the variability of temperature tropism among the groups of mice which was treated with MHD, MXSGD, and their compositions. Meanwhile, the total anti-oxidant capability (T-AOC) activity were detected. RESULTS:After administration of MHD, the mice showed increased oxygen consumption (P<0.01). Compared with MHD group, the remaining rate of MXSGD mice on the hot pad was found to be significantly increased with the cold-hot pad differentiating assay (P<0.05). There was no significant difference (P>0.05) among the remaining rates of MXSGD, MXSGD with high dose Gypsum Fibrosum (MXHGF) group, and MXSGD with low dose Gypsum Fibrosum (MXLGF) group mice. Compared with the MHD group, T-AOC activity of the mice in the Consensus Compositons group was significantly decreased (P=0.0494). Compared with the MXSGD group, T-AOC activity of Gypsum Fibrosum (GF) group was increased significantly (P=0.0013). CONCLUSIONS:The differences in cold and hot nature could be represented objectively between MHD with a hot nature and MXSGD with a cold nature. The reason may be the Gypsum Fibrosum which decreased the efficacy of the consensus compositions. However, increasing or decreasing the dose of Gypsum Fibrosum will not change the cold and hot nature of MXSGD. 10.1007/s11655-011-0694-2

Multisolvent Similarity Measure of Chinese Herbal Medicine Ingredients for Cold-Hot Nature Identification. Wei Guohui,Fu Xianjun,Wang Zhenguo Journal of chemical information and modeling Cold-hot nature theory is the core basic theory of traditional Chinese medicine (TCM). "Treating the hot syndrome with cold nature medicine and treating cold syndrome with hot nature medicine" indicates that correct classification of medical properties (cold or hot nature) of Chinese herbal medicines (CHMs) is an important basis for TCM treatment. In this study, we propose a novel multisolvent similarity measure retrieval scheme (MSSMRS) for discriminating CHMs as cold or hot. We explore a multisolvent distance metric learning algorithm to calculate similarity measure of CHM ingredients, and a retrieval scheme for nature identification. First, four solvents (chloroform, distilled water, absolute ethanol, and petroleum ether) are applied to extract ultraviolet (UV) spectrum data of CHM ingredients. Second, we study quantifying the similarity of CHM ingredients to fingerprint similarity. We explore a multisolvent distance metric learning (MSDML) algorithm to measure the similarity of CHM ingredients. MSDML can discover complementary characteristics of different solvent data sets through an optimization algorithm. Finally, a retrieval scheme is designed to analyze the relationship between the CHM ingredients and cold-hot nature. Extensive experimental results demonstrate that CHMs with similar compositions of substances have similar medicinal natures. Experimental evaluations based on the proposed retrieval scheme suggest the effectiveness of MSDML in the identification of the nature of CHMs. 10.1021/acs.jcim.9b00682