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Alcian blue/alizarin red staining of cartilage and bone in mouse. Ovchinnikov Dmitry Cold Spring Harbor protocols 10.1101/pdb.prot5170
Whole-mount skeletal staining. Rigueur Diana,Lyons Karen M Methods in molecular biology (Clifton, N.J.) The first step in almost every investigation of skeletal phenotypes is analysis of whole-mount skeletal preparations. Whole-mount skeletal staining permits evaluation of the shapes and sizes of skeletal elements in their appropriate locations. The technique is thus the major method for detecting changes in skeletal patterning. Because cartilage and bone can be distinguished by differential staining, this technique is also a powerful means to assess the pace of skeletal maturation. This protocol covers staining of the pre- and postnatal mouse skeleton using Alcian blue and Alizarin red to identify cartilage and bone, respectively. 10.1007/978-1-62703-989-5_9