1. Antibodies against viral nucleo-, phospho-, and X protein contribute to serological diagnosis of fatal Borna disease virus 1 infections.
作者:Neumann Bernhard , Angstwurm Klemens , Linker Ralf A , Knoll Gertrud , Eidenschink Lisa , Rubbenstroth Dennis , Schlottau Kore , Beer Martin , Schreiner Patrick , Soutschek Erwin , Böhmer Merle M , Lampl Benedikt M J , Pregler Matthias , Scheiter Alexander , Evert Katja , Zoubaa Saida , Riemenschneider Markus J , Asbach Benedikt , Gessner André , Niller Hans Helmut , Schmidt Barbara , Bauswein Markus
期刊:Cell reports. Medicine
日期:2022-01-18
DOI :10.1016/j.xcrm.2021.100499
Borna disease virus 1 (BoDV-1) causes rare but often fatal encephalitis in humans. Late diagnosis prohibits an experimental therapeutic approach. Here, we report a recent case of fatal BoDV-1 infection diagnosed on day 12 after hospitalization by detection of BoDV-1 RNA in the cerebrospinal fluid. In a retrospective analysis, we detect BoDV-1 RNA 1 day after hospital admission when the cell count in the cerebrospinal fluid is still normal. We develop a new ELISA using recombinant BoDV-1 nucleoprotein, phosphoprotein, and accessory protein X to detect seroconversion on day 12. Antibody responses are also shown in seven previously confirmed cases. The individual BoDV-1 antibody profiles show variability, but the usage of three different BoDV-1 antigens results in a more sensitive diagnostic tool. Our findings demonstrate that early detection of BoDV-1 RNA in cerebrospinal fluid and the presence of antibodies against at least two different viral antigens contribute to BoDV-1 diagnosis. Physicians in endemic regions should consider BoDV-1 infection in cases of unclear encephalopathy and initiate appropriate diagnostics at an early stage.
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2区Q1影响因子: 5.7
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2. Membranoproliferative Glomerulonephritis With Changing Immunofluorescence Pattern.