Betaine in Inflammation: Mechanistic Aspects and Applications.
Frontiers in immunology
Betaine is known as trimethylglycine and is widely distributed in animals, plants, and microorganisms. Betaine is known to function physiologically as an important osmoprotectant and methyl group donor. Accumulating evidence has shown that betaine has anti-inflammatory functions in numerous diseases. Mechanistically, betaine ameliorates sulfur amino acid metabolism against oxidative stress, inhibits nuclear factor-κB activity and NLRP3 inflammasome activation, regulates energy metabolism, and mitigates endoplasmic reticulum stress and apoptosis. Consequently, betaine has beneficial actions in several human diseases, such as obesity, diabetes, cancer, and Alzheimer's disease.
10.3389/fimmu.2018.01070
Chitosan-based injectable hydrogel with multifunction for wound healing: A critical review.
Carbohydrate polymers
Different types of clinical wounds are difficult to treat while infected by bacteria. Wound repair involves multiple cellular and molecular interactions, which is a complicated process. However, wound repair often suffers from abnormal cellular functions or pathways that result in unavoidable side effects, so there is an urgent need for a material that can heal wounds quickly and with few side effects. Based on these needs, hydrogels with injectable properties have been confirmed to be able to undergo self-healing, which provides favorable conditions for wound healing. Notably, as a biopolymer with excellent easy-to-modify properties from a wide range of natural sources, chitosan can be used to prepare injectable hydrogel with multifunction for wound healing because of its outstanding flowability and injectability. Especially, chitosan-based hydrogels with marked biocompatibility, non-toxicity, and bio-adhesion properties are ideal for facilitating wound healing. In this review, the characteristics and healing mechanisms of different wounds are briefly summarized. In addition, the preparation and characterization of injectable chitosan hydrogels in recent years are classified. Additionally, the bioactive properties of this type of hydrogel in vitro and in vivo are demonstrated, and future trend in wound healing is prospected.
10.1016/j.carbpol.2024.121952
Chitosan, alginate, hyaluronic acid and other novel multifunctional hydrogel dressings for wound healing: A review.
International journal of biological macromolecules
Wound healing is a complex project, and effectively promoting skin repair is a huge clinical challenge. Hydrogels have great prospect in the field of wound dressings because their physical properties are very similar to those of living tissue and have excellent properties such as high water content, oxygen permeability and softness. However, the single performance of traditional hydrogels limits their application as wound dressings. Therefore, natural polymers such as chitosan, alginate and hyaluronic acid, which are non-toxic and biocompatible, are individually or combined with other polymer materials, and loaded with typical drugs, bioactive molecules or nanomaterials. Then, the development of novel multifunctional hydrogel dressings with good antibacterial, self-healing, injectable and multi-stimulation responsiveness by using advanced technologies such as 3D printing, electrospinning and stem cell therapy has become a hot topic of current research. This paper focuses on the functional properties of novel multifunctional hydrogel dressings such as chitosan, alginate and hyaluronic acid, which lays the foundation for the research of novel hydrogel dressings with better performance.
10.1016/j.ijbiomac.2023.124321
Polysaccharide-Based Multifunctional Hydrogel Bio-Adhesives for Wound Healing: A Review.
Gels (Basel, Switzerland)
Wound healing is a long-term and complex biological process that involves multiple hemostasis, inflammation, proliferation, and remodeling stages. In order to realize comprehensive and systematic wound management, appropriate wound treatment bio-adhesives are urgently needed. Hydrogel bio-adhesives have excellent properties and show unique and remarkable advantages in the field of wound management. This review begins with a detailed description of the design criteria and functionalities of ideal hydrogel bio-adhesives for wound healing. Then, recent advances in polysaccharide-based multifunctional hydrogel bio-adhesives, which involve chitosan, hyaluronic acid, alginate, cellulose, dextran, konjac glucomannan, chondroitin sulfate, and other polysaccharides, are comprehensively discussed. Finally, the current challenges and future research directions of polysaccharide-based hydrogel bio-adhesives for wound healing are proposed to stimulate further exploration by researchers.
10.3390/gels9020138
Biological macromolecule-based hydrogels with antibacterial and antioxidant activities for wound dressing: A review.
International journal of biological macromolecules
Because of the complex symptoms resulting from metabolic dysfunction in the wound microenvironment during bacterial infections, along with the necessity to combat free radicals, achieving prompt and thorough wound healing remains a significant medical challenge that has yet to be fully addressed. Moreover, the misuse of common antibiotics has contributed to the emergence of drug-resistant bacteria, underscoring the need for enhancements in the practical and commonly utilized approach to wound treatment. In this context, hydrogel dressings based on biological macromolecules with antibacterial and antioxidant properties present a promising new avenue for skin wound treatment due to their multifunctional characteristics. Despite the considerable potential of this innovative approach to wound care, comprehensive research on these multifunctional dressings is still insufficient. Consequently, the development of advanced biological macromolecule-based hydrogels, such as chitosan, alginate, cellulose, hyaluronic acid, and others, has been the primary focus of this study. These materials have been enriched with various antibacterial and antioxidant agents to confer multifunctional attributes for wound healing purposes. This review article aims to offer a comprehensive overview of the latest progress in this field, providing a critical theoretical basis for future advancements in the utilization of these advanced biological macromolecule-based hydrogels for wound healing.
10.1016/j.ijbiomac.2024.134578
Carboxymethyl Chitosan/Sodium Alginate/Chitosan Quaternary Ammonium Salt Composite Hydrogel Supported 3J for the Treatment of Oral Ulcer.
Gels (Basel, Switzerland)
Oral ulcer is a common inflammatory disease of oral mucosa, causing severe burning pain and great inconvenience to daily life. In this study, compound with anti-inflammatory activity was synthesized beforehand. Following that, an intelligent composite hydrogel supported was designed with sodium alginate, carboxymethyl chitosan, and chitosan quaternary ammonium salt as the skeleton, and its therapeutic effect on the rat oral ulcer model was investigated. The results show that the composite hydrogel has a dense honeycomb structure, which is conducive to drug loading and wound ventilation, and has biodegradability. It has certain antibacterial effects and good anti-inflammatory activity. When loaded with , it reduced levels of TNF-α and IL-6 in inflammatory cells by up to 50.0%. It has excellent swelling and water retention properties, with a swelling rate of up to 765.0% in a pH 8.5 environment. The existence of a large number of quaternary ammonium groups, carboxyl groups, and hydroxyl groups makes it show obvious differences in swelling in different pH environments, which proves that it has double pH sensitivity. It is beneficial to adapt to the highly dynamic changes of the oral environment. Compared with single hydrogel or drug treatment, the drug-loaded hydrogel has a better effect on the treatment of oral ulcers.
10.3390/gels9080659
Preparation and characterization of a dual cross-linking injectable hydrogel based on sodium alginate and chitosan quaternary ammonium salt.
Carbohydrate research
The development of cheap and easily available injectable hydrogel is an urgent problem in the field of biomedical engineering. Herein, we used chitosan quaternary ammonium salt and sodium alginate to prepare a dual crosslinking hydrogel. The hydrogel formed in-situ crosslinking and can be injected continuously. Interestingly, the formed hydrogel possessed a homogeneous 3D network structure and exhibited reasonable mechanical properties. Moreover, the hydrogels had excellent injectability, and the compression strength of the hydrogel (Gel-0.5) was up to 27.65 kPa. Additionally, the hydrogel showed good biocompatibility that evaluated by cytotoxicity. Notably, the hydrogel was nontoxic toward NIH-3T3 cells. In summary, the hydrogel we produced can be used as an ideal biomaterial for further applications in the field of biomedical engineering.
10.1016/j.carres.2021.108389
Study on polysaccharide polyelectrolyte complex and fabrication of alginate/chitosan derivative composite fibers.
Zhao Tongyao,Li Xiaoyan,Gong Yumei,Guo Yanzhu,Quan Fengyu,Shi Qiang
International journal of biological macromolecules
Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers. The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter. The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change. Moreover, the electrical potential also depended on salt in solution. By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process. The FT-IR and EDS determination indicated there formed egg-box between SA and Ca, cross-linked network between glutaraldehyde(GA) and SA, QAC, respectively. Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.
10.1016/j.ijbiomac.2021.05.150
Multifunctional double-network hydrogel with antibacterial and anti-inflammatory synergistic effects contributes to wound healing of bacterial infection.
International journal of biological macromolecules
Wound infection not only hinders the time sequence of tissue repair, but also may lead to serious complications. Multifunctional wound dressings with biocompatibility, excellent mechanical properties and antibacterial properties can promote wound healing during skin infection and reduce the use of antibiotics. In this study, a multifunctional dual-network antibacterial hydrogel was constructed based on the electrostatic interaction of two polyelectrolytes, hydroxypropyl trimethyl ammonium chloride chitosan (HACC) and sodium alginate (SA). Attributing to the suitable physical crosslinking between HACC and SA, the hydrogel not only has good biocompatibility, mechanical property, but also has broad-spectrum antibacterial properties. In vivo results showed that the hydrogel could regulate M2 polarization, promote early vascular regeneration, and create a good microenvironment for wound healing. Therefore, this hydrogel is an effective multifunctional wound dressing. Consequently, we propose a novel hydrogel with combined elements to expedite the intricate repair of wound infection.
10.1016/j.ijbiomac.2024.132672
Gallic acid: design of a pyrogallol-containing hydrogel and its biomedical applications.
Biomaterials science
Polyphenol hydrogels have garnered widespread attention due to their excellent adhesion, antioxidant, and antibacterial properties. Gallic acid (GA) is a typical derivative of pyrogallol that is used as a hydrogel crosslinker or bioactive additive and can be used to make multifunctional hydrogels with properties superior to those of widely studied catechol hydrogels. Furthermore, compared to polymeric tannic acid, gallic acid is more suitable for chemical modification, thus broadening its range of applications. This review focuses on multifunctional hydrogels containing GA, aiming to inspire researchers in future biomaterial design. We first revealed the interaction mechanisms between GA molecules and between GA and polymers, analyzed the characteristics GA imparts to hydrogels and compared GA hydrogels with hydrogels containing catechol. Subsequently, in this paper, various methods of integrating GA into hydrogels and the applications of GA in biomedicine are discussed, finally assessing the current limitations and future development potential of GA. In summary, GA, a natural small molecule polyphenol with excellent functionality and diverse interaction modes, has great potential in the field of biomedical hydrogels.
10.1039/d3bm01925j