Genome-Wide Identification and Expression Analysis of the Copper Transporter (/) Gene Family in , a Typical Mangrove Plant. International journal of molecular sciences The copper transporter (/) gene family plays a critical part in maintaining the balance of the metal, and many diverse species depend on to move copper (Cu) across the cell membrane. In , , , , , , and a genome-wide study of the COPT protein family was performed. To understand the major roles of the gene family in (), a genome-wide study identified four genes in the genome for the first time. The domain and 3D structural variation, phylogenetic tree, chromosomal distributions, gene structure, motif analysis, subcellular localization, cis-regulatory elements, synteny and duplication analysis, and expression profiles in leaves and Cu were all investigated in this research. Structural and sequence investigations show that most have three transmembrane domains (TMDs). According to phylogenetic research, these might be divided into two subgroups, just like . gene segmental duplications and positive selection pressure were discovered by universal analysis. According to gene structure and motif analysis, most genes showed consistent exon-intron and motif organization within the same group. In addition, we found five hormones and four stress- and seven light-responsive cis-elements in the promoters. The expression studies revealed that all four genes changed their expression levels in response to copper (CuCl) treatments. In summary, our study offers a thorough overview of the gene family's expression pattern and functional diversity, making it easier to characterize each gene's function in the future. 10.3390/ijms242115579

Molecular Characterization of Gene Family in . Li Xiu-Yun,Lin Er-Pei,Huang Hua-Hong,Niu Ming-Yue,Tong Zai-Kang,Zhang Jun-Hong Frontiers in plant science As a major family of plant-specific transcription factors, () genes play vital regulatory roles in plant growth, development and stress responses. In this study, 18 genes were identified and cloned from . Two zinc finger-like structures and a nuclear location signal (NLS) segments were existed in the SBP domains of all BlSPLs. Phylogenetic analysis showed that these genes were clustered into nine groups (group I-IX). The intron/exon structure and motif composition were highly conserved within the same group. 12 of the 18 s were experimentally verified as the targets of miR156, and two cleavage sites were detected in these miR156-targeted genes. Many putative cis-elements, associated with light, stresses and phytohormones response, were identified in the promoter regions of s, suggesting that genes are probably involved in important physiological processes and developmental events. Tissue-specific expression analysis showed that miR156-targeted s exhibited a more differential expression pattern, while most miR156-nontargeted s tended to be constitutively expressed, suggesting the distinct roles of miR156-targeted and nontargeted s in development and growth of . Further expression analysis revealed that miR156-targeted s were dramatically up-regulated with age, whereas mature BlmiR156 level was apparently declined with age, indicating that miR156/ module plays important roles in vegetative phase change of . Moreover, yeast two-hybrid assay indicated that several miR156-targeted and nontargeted BlSPLs could interact with two DELLA proteins (BlRGA and BlRGL), which suggests that certain s take part in the GA regulated processes through protein interaction with DELLA proteins. All these results provide an important basis for further exploring the biological functions of s in . 10.3389/fpls.2018.00608

The Gene Family in : Genome-Wide Identification, Expression Profiles, and Functional Interaction Network Analysis. Frontiers in plant science Xyloglucan endotransglucosylase/hydrolase (XTH), belonging to glycoside hydrolase family 16, is one of the key enzymes in plant cell wall remodeling. is an important timber and fireproof tree species in southern China. However, little is known about in . In the present study, a total of 34 were obtained, which were classified into three subfamilies based on the phylogenetic relationship and unevenly distributed on 18 chromosomes. Furthermore, the intron-exon structure and conserved motif composition of them supported the classification and the members belonging to the same subfamily shared similar gene structures. Segmental and tandem duplication events did not lead to gene family expansion, and strong purifying selection pressures during evolution led to similar structure and function of gene family. The interaction network and -acting regulatory elements analysis revealed the expression might be regulated by multiple hormones, abiotic stresses and transcription factors. Finally, expression profiles and GO enrichment analysis showed most of the tandem repeat genes were mainly expressed in the phloem and xylem and they mainly participated in glycoside metabolic processes through the transfer and hydrolysis of xyloglucan in the cell wall and then regulated fiber elongation. 10.3389/fpls.2022.911761