Low energy treatment of landfill leachate using simultaneous partial nitrification and partial denitrification with anaerobic ammonia oxidation.
Wu Lina,Li Zhi,Huang Shan,Shen Mingyu,Yan Zhibin,Li Jin,Peng Yongzhen
Environment international
An up-flow anaerobic sludge blanket reactor (UASB), anoxic/oxic (A/O)-anaerobic ammonia oxidation reactor (ANAOR or anammox reactor), and anaerobic sequencing batch reactor (ASBR) were employed in the treatment of landfill leachate with partial nitrification-anammox and half-denitrification-anammox. The Chemical Oxygen Demand (COD) concentration, ammonium nitrogen (NH-N) concentration, and total nitrogen (TN) concentration of the basal leachate was 2200-2500 mg/L, 1200-1300 mg/L, and 1300-1400 mg/L, respectively. After a 1:2 dilution using domestic sewage, the COD, NH-N, and TN concentrations in the influent were 800-1000 mg/L, 400-430 mg/L, and 420-440 mg/L, respectively. After treatment, the final COD, NH-N, and TN were decreased to 90-100 mg/L, 13-14 mg/L, and 35-38 mg/L, respectively. In the ASBR, organic carbon sources in sewage-diluted landfill leachate were introduced for the conversion of nitrate nitrogen (NO-N) into nitrite nitrogen (NO-N). This enabled the continued reaction of NO-N with NH-N from the newly introduced sewage-diluted landfill leachate via anammox. As a result, complete TN removal was achieved in the system. Microbial diversity analysis indicated that the relative abundance of ammonia-oxidizing bacteria (AOB) was four to five times greater than nitrite-oxidizing bacteria (NOB) in the A/O reactor, showing that partial nitrification was prevalent. The relative abundance of the anammox bacterium Candidatus Kuenenia gradually increased in each reactor, reaching a maximum of 1.17%-1.39%. Using this set-up, we achieved advanced, efficient, and economical, COD reduction and nitrogen removal. Taken together, the findings provide important insights into the optimal operation of landfill leachate treatments.
10.1016/j.envint.2019.02.071
Achieving high nitrogen removal efficiency by optimizing nitrite-dependent anaerobic methane oxidation process with growth factors.
Wang Jiaqi,Hua Miaolian,Li Yufen,Ma Fang,Zheng Ping,Hu Baolan
Water research
Nitrite-dependent anaerobic methane oxidation (N-DAMO) is a newly discovered bioprocess which uses methane as electron donor to reduce nitrite into dinitrogen. It is a promising clean bioprocess for denitrification in wastewater treatment. However, the low reaction rate and slow growth rate of N-DAMO bacteria within NC10 phylum limit the application of the process. In this study, we chose vitamin, heme, nucleobase and betaine to investigate their short- and long-term effects on N-DAMO bacteria. The concentrations of the growth factors of medium were improved according to the short-term experiments. The results were subsequently verified via long-term inoculations and were applied in a magnetically stirred gas lift reactor (MSGLR). The results indicated that nucleobase and betaine (5.0 and 200 μg L, respectively) significantly stimulated the N-DAMO activity, whereas vitamin and heme had no significant effects in the tested concentration ranges. During the long-term incubation, N-DAMO bacteria continuously increased and finally achieved a relative abundance of 14.4% on day 300. Notably, larger aggregates of N-DAMO bacteria were observed at the end of the long-term incubation. And the nitrogen removal rate of the MSGLR increased to 70 mg N L day, with the total nitrogen removal efficiency over 99.0%. However, the addition of betaine introduced methyl into the reactors and this made methylotrophs account a considerable part of the bacterial community, which limited the enrichment degree of N-DAMO bacteria. This work will contribute to the engineering application and enrichment of N-DAMO bacteria.
10.1016/j.watres.2019.05.101
Simultaneous Ammonium oxidation denitrifying (SAD) in an innovative three-stage process for energy-efficient mature landfill leachate treatment with external sludge reduction.
Zhang Fangzhai,Li Xiyao,Wang Zhong,Jiang Hao,Ren Shang,Peng Yongzhen
Water research
High-loaded ammonia and low-strength organics mature landfill leachate is not effectively treated by conventional biological processes. Herein, an innovative solution was proposed using a three-stage Simultaneous Ammonium oxidation Denitrifying (SAD) process. Firstly, ammonia (1760 ± 126 mg N/L) in wastewater was oxidized to nitrite in a partial nitrification sequencing batch reactor (PN-SBR). Next, 93% PN-SBR effluent and concentrated external waste activated sludge (WAS; MLSS = 23057 ± 6014 mg/L) were introduced to an anoxic reactor for integrated fermentation and denitrification (IFD-SBR). Finally, ammonia (101.4 ± 13.8 mg N/L) released by fermentation in the IFD-SBR and residual 7% nitrite in the PN-SBR were removed through the anaerobic ammonium oxidation (anammox) process in the SAD up-flow anaerobic sludge bed (SAD-UASB). In addition, NO-N generation during the anammox process could be reduced to nitrite by partial denitrification (PD) and reused as substrate for anammox. A satisfactory total nitrogen (TN) removal efficiency (98.3%), external sludge reduction rate (2.5 kg/m d) and effluent TN concentration (16.7 mg/L) were achieved after long-term operation (280 days). The IFD-SBR and SAD-UASB contributed to 81.9% and 12.3% nitrogen removal, respectively. Microbial analysis showed that anammox bacteria (1.5% Candidatus Brocadia) cooperated well with partial denitrifying bacteria (4.3% Thauera) in SAD-UASB, and average nitrogen removal contribution were 83.1% during significant stability of anammox and 9.2% during the denitrification process, respectively. The three-stage SAD process provides an environmental and economic approach for landfill leachate treatment since it has the advantage of 25.4% less oxygen, 100% organic matter savings and 47.9% less external sludge than traditional biological processes.
10.1016/j.watres.2019.115156
Operation strategies of n-DAMO and Anammox process based on microbial interactions for high rate nitrogen removal from landfill leachate.
Nie Wen-Bo,Xie Guo-Jun,Ding Jie,Peng Lai,Lu Yang,Tan Xin,Yue Hao,Liu Bing-Feng,Xing De-Feng,Meng Jia,Han Hong-Jun,Ren Nan-Qi
Environment international
Nitrate/nitrite-dependent anaerobic methane oxidation (n-DAMO) coupling to Anaerobic ammonium oxidation (Anammox) provides an opportunity for simultaneous nitrogen removal and methane emissions mitigation from wastewater. However, to achieve high nitrogen removal rate in such a process remains a critical challenge in practical application. This work investigated the interactions between n-DAMO and Anammox in membrane biofilm reactor (MBfR) and then developed operational strategies of MBfR for high rate nitrogen removal from landfill leachate. Initially, influent containing nitrate and ammonium facilitated the development of n-DAMO and Anammox microorganisms in MBfR, but nitrogen removal performance is hard to be further improved even deteriorated. Detailed investigations of interactions among n-DAMO and Anammox microorganisms confirmed that extra addition of nitrite into MBfR fed with nitrate and ammonium not only stimulated the activities of Anammox bacteria, but also enhanced the activities of n-DAMO archaea from 172.3 to 356.9 mg NO-N L d. Functional gene analysis also indicated that mcrA and hzsA genes increased after nitrite addition. Based on this finding, influent containing NO, NO and NH enabled nitrogen removal rates of MBfR increase from 224.9 to 888.2 mg N L d. Finally, nitrate in the influent was gradually replaced with nitrite to mimic the effluent from partial nitriation of landfill leachate, but maintain the nitrate availability for n-DAMO archaea through increasing nitrate production from Anammox. These operation strategies enabled MBfR achieve the steady state with a nitrogen removal rate of 6.1 kg N m d. Microbial community analysis revealed n-DAMO archaea, n-DAMO bacteria and Anammox bacteria jointly dominated the biofilm, and their relative abundance dynamically shifted with feeding regime. This work provides promising operational strategies for high rate of nitrogen removal from landfill leachate through integrating n-DAMO and Anammox process.
10.1016/j.envint.2020.105596
Strategy for denitrifying anaerobic methane-oxidizing bacteria growing under the oxygen-present condition.
Li Yufen,Wang Jiaqi,Hua Miaolian,Yao Xiangwu,Zhao Yuxiang,Hu Jiajie,Xi Chuanwu,Hu Baolan
The Science of the total environment
Denitrifying anaerobic methane oxidizing (DAMO) bacteria are newly discovered microorganisms that use methane as the electron donor to reduce nitrite into dinitrogen. They have potential value on nitrogen removal from wastewater. However, the oxygen exposure in engineering is considered one of the bottlenecks for DAMO engineering application. In this work, we cultured DAMO bacteria under oxic and anoxic conditions in a gas-lift sequencing batch reactor (GLSBR) to explore DAMO bacterial response to oxygen stress. Under oxic conditions (7.5-8 mg O/L), the extension of hydraulic retention time (HRT) from 2 days to 4 days increased DAMO bacterial abundance by 3.8 times. Under anoxic conditions (0.2-0.5 mg O/L), DAMO bacterial abundance increased by 30.1 times and were kept over 2.0 × 10 copies g wet sludge. During the enrichment, microbial aggregates were formed and DAMO bacteria tended to be distributed inside the aggregates. Notably, aerobic methanotrophs existed in the whole process, capable of consuming oxygen and providing a suitable environment for DAMO bacterial growth. Finally, DAMO bacteria were enriched and the relative abundance was 16.16%. This work provides new insights into DAMO bacterial enrichment and their application in wastewater treatment.
10.1016/j.scitotenv.2020.140476