Performance Assessment of Internal Quality Control (IQC) Products in Blood Transfusion Compatibility Testing in China.
Xu Gui-Ping,Wu Li-Fang,Li Jing-Jing,Gao Qi,Liu Zhi-Dong,Kang Qiong-Hua,Hou Yi-Jun,Zhang Luo-Chuan,Hu Xiao-Mei,Li Jie,Zhang Juan
Internal quality control (IQC) is a critical component of laboratory quality management, and IQC products can determine the reliability of testing results. In China, given the fact that most blood transfusion compatibility laboratories do not employ IQC products or do so minimally, there is a lack of uniform and standardized IQC methods. To explore the reliability of IQC products and methods, we studied 697 results from IQC samples in our laboratory from 2012 to 2014. The results showed that the sensitivity and specificity of the IQCs in anti-B testing were 100% and 99.7%, respectively. The sensitivity and specificity of the IQCs in forward blood typing, anti-A testing, irregular antibody screening, and cross-matching were all 100%. The reliability analysis indicated that 97% of anti-B testing results were at a 99% confidence level, and 99.9% of forward blood typing, anti-A testing, irregular antibody screening, and cross-matching results were at a 99% confidence level. Therefore, our IQC products and methods are highly sensitive, specific, and reliable. Our study paves the way for the establishment of a uniform and standardized IQC method for pre-transfusion compatibility testing in China and other parts of the world.
Large transport J(c) in Cu-sheathed Sr(0.6)K(0.4)Fe2As2 superconducting tape conductors.
Lin He,Yao Chao,Zhang Haitao,Zhang Xianping,Zhang Qianjun,Dong Chiheng,Wang Dongliang,Ma Yanwei
Copper sheath is the first choice for manufacturing high-T(c) superconducting wires and tapes because of its high electrical and thermal conductivities, low-cost and good mechanical properties. However, Cu can easily react with superconducting cores, such as BSCCO, MgB2 and pnictides, and therefore drastically decrease the transport J(c). Here, we report the fabrication of Cu-sheathed Sr(1-x)K(x)Fe2As2 tapes with superior J(c) performance using a simple hot pressing method that is capable of eliminating the lengthy high-temperature sintering. We obtained high-quality Sr(1-x)K(x)Fe2As2 tapes with processing at 800 °C for 30 minutes and measured high T(c) and sharp transition. By this rapid fabrication, Cu sheath does not give rise to apparent reaction layer, and only slightly diffuses into Sr-122 core. As a consequence, we achieved high transport J(c) of 3.1 × 10(4) A/cm(2) in 10 T and 2.7 × 10(4) A/cm(2) in 14 T at 4.2 K. The in-field J(c) performance is by far the highest reported for Cu-sheathed high-T(c) conductors. More importantly, Cu-sheathed Sr-122 tapes also showed a high J(e) value of 1.0 × 10(4) A/cm(2) in 10 T at 4.2 K, which has reached the widely accepted practical level for applications. These results demonstrate that Cu is a very promising sheath for the practical application of pnictide conductors.
Lentivirus-mediated IL-10-expressing Bone Marrow Mesenchymal Stem Cells promote corneal allograft survival upregulating lncRNA 003946 in a rat model of corneal allograft rejection.
Lu Xiaoxiao,Ru Yusha,Chu Chenchen,Lv Ying,Gao Yichen,Jia Zhe,Huang Yue,Zhang Yan,Zhao Shaozhen
Corneal transplantation is an effective treatment to corneal blindness. However, the immune rejection imperils corneal allograft survival. An interventional modality is urgently needed to inhibit immune rejection and promote allograft survival. In our previous study, subconjunctival injections of bone marrow-derived mesenchymal stem cells (BM-MSCs) into a rat model of corneal allograft rejection extended allograft survival for 2 d. In this study, we sought to generate IL-10-overexpressing BM-MSCs, aiming to boost the survival-promoting effects of BM-MSCs on corneal allografts and explore the molecular and cellular mechanisms underlying augmented protection. A population of IL-10-overexpressing BM-MSCs (designated as IL-10-BM-MSCs) were generated by lentivirus transduction and FACS purification. The self-renewal, multi-differentiation, and immunoinhibitory capabilities of IL-10-BM-MSCs were examined by conventional assays. The IL-10-BM-MSCs were subconjunctivally injected into the model of corneal allograft rejection, and the allografts were monitored on a daily basis. The expression profiling of long noncoding RNA (lncRNA) in the allografts was revealed by RNA sequencing and verified by quantitative real-time PCR. The infiltrating immune cell type predominantly upregulating the lncRNA expression was identified by RNAscope hybridization. The function of the upregulated lncRNA was proved by loss- and gain-of-function experiments both and . The IL-10-BM-MSCs possessed an enhanced immunoinhibitory capability and unabated self-renewal and multi-differentiation potentials as compared to plain BM-MSCs. The subconjunctivally injected IL-10-BM-MSCs reduced immune cell infiltration and doubled allograft survival time (20 d) as compared to IL-10 protein or plain BM-MSCs in the corneal allograft rejection model. Further, IL-10-BM-MSCs significantly upregulated lncRNA 003946 expression in CD68 macrophages infiltrating corneal allografts. Silencing and overexpressing lncRNA 003946 in macrophage cultures abolished and mimicked the IL-10-BM-MSCs' suppressing effects on the macrophages' antigen presentation, respectively. In parallel, knocking down and overexpressing the lncRNA abrogated and simulated the survival-promoting effects of IL-10-BM-MSCs on corneal allografts, respectively. The remarkable protective effects of IL-10-BM-MSCs support further developing them into an effective interventional modality against corneal allograft rejection. IL-10-BM-MSCs promote corneal allograft survival mainly through upregulating a novel lncRNA expression in graft-infiltrating CD68 macrophages. LncRNA, for the first time, is integrated into an IL-10-BM-MSC-driven immunomodulatory axis against the immune rejection to corneal allograft.