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Functional Silver-Silicone-Nanofilament-Composite Material for Water Disinfection. Meier Margrith,Suppiger Angela,Eberl Leo,Seeger Stefan Small (Weinheim an der Bergstrasse, Germany) The roughness of superhydrophobic silicone nanofilaments (SNFs) is exploited to enlarge the contact area of conventional filter material. As an efficient wetting of the filter material is crucial for water treatment, the wettability of SNFs is readily modified from superhydrophobic to hydrophilic during the functionalization process. SNFs are coated on glass beads and subsequently modified with biocidal silver nanoparticles (AgNPs). The enlarged surface area of SNFs allows a 30 times higher loading of AgNPs in comparison to glass beads without SNF coating. Thus, in column experiments, the AgNP-SNF-nanocomposite-modified glass beads exert superior antibacterial activity towards suspensions of E. coli K12 compared to AgNP functionalized glass beads without SNFs. Additionally, reusing the AgNP-SNF-nanocomposite-coated glass beads with fresh bacteria contaminated medium increases their efficacy and reduces the colony forming units by ≈6 log units. Thereby, the silver loss during percolation is below 0.1 μg mL . These results highlight, first, the potential of AgNP-SNF-nanocomposite-modified glass beads as an effective filter substrate for water disinfection, and second, the efficiency of SNF coating in increasing the contact area of conventional filter material. 10.1002/smll.201601072
Dually functional polyethylenimine-coated gold nanoparticles: a versatile material for electrode modification and highly sensitive simultaneous determination of four tumor markers. Putnin Thitirat,Ngamaroonchote Aroonsri,Wiriyakun Natta,Ounnunkad Kontad,Laocharoensuk Rawiwan Mikrochimica acta A novel sandwich-type electrochemical multiplex immunoassay is described for simultaneous detection of the tumor biomarkers alpha fetoprotein (AFP), carcinoembryonic antigen (CEA), prostate-specific antigen (PSA) and interleukin-8 (IL-8). Polyethylenimine-coated gold nanoparticles (PEI-AuNPs) were used for both modification of a screen-printed carbon electrode (SPCE) and as labeling tags. The coated AuNPs can be easily adsorbed on the electrodes which also are loaded with the electroactive metal ions cadmium(II), lead(II) copper(II) and silver(I) and related secondary antibodies (Ab). These give distinct voltammetric signals at -0.80, -0.55, -0.20 and + 0.05 V, respectively (vs Ag/AgCl). Four corresponding capture antibodies (Ab) were then conjugated to one of the electrodes. After a sandwich-type structure was formed by binding of the analytes and the labeling AuNPs, the electrochemical signal responses were recorded. Under the optimized testing conditions, there is a linear relationship in range from 0.25-10 ng mL for AFP, CEA and PSA, and from 0.50-100 pg mL for IL-8. The corresponding detection limits are 1.7, 1.6, 0.9 and 1.0 fg mL, respectively. Cross reactivity, interferences and stability of the modified electrodes and of the signal nanotags are satisfying in that they can be stored for >4 weeks without significant signal reduction. The method was successfully applied to the determination of the biomarkers in spiked human serum. Graphical abstract Poly(ethylenimine)-coated gold nanoparticles were used in a sandwich-type multiplex electrochemical immunosensor. The coated gold nanoparticles were used for both electrode modification and as electrochemical nanotags. The resultingvmmunosensor exhibits excellent sensitivity for the four analytes studied, and also displays selectivity and long-term stability. 10.1007/s00604-019-3370-4
Supramolecular biomaterials. Webber Matthew J,Appel Eric A,Meijer E W,Langer Robert Nature materials Polymers, ceramics and metals have historically dominated the application of materials in medicine. Yet rationally designed materials that exploit specific, directional, tunable and reversible non-covalent interactions offer unprecedented advantages: they enable modular and generalizable platforms with tunable mechanical, chemical and biological properties. Indeed, the reversible nature of supramolecular interactions gives rise to biomaterials that can sense and respond to physiological cues, or that mimic the structural and functional aspects of biological signalling. In this Review, we discuss the properties of several supramolecular biomaterials, as well as their applications in drug delivery, tissue engineering, regenerative medicine and immunology. We envision that supramolecular biomaterials will contribute to the development of new therapies that combine highly functional materials with unmatched patient- and application-specific tailoring of both material and biological properties. 10.1038/nmat4474