加载中

    Antiviral activity of phosphorylated Radix Cyathulae officinalis polysaccharide against Canine Parvovirus in vitro. Feng Haibo,Fan Jing,Yang Shiping,Zhao Xuelian,Yi Xiao International journal of biological macromolecules Phosphorylated Radix Cyathulae officinalis Kuan polysaccharides (pRCPS) was prepared according to three-factors, ratio of STMP (%) and STPP (%), reaction time and reaction temperature, and three level L(3) orthogonal design. The antiviral activity of nine pRCPS (pRCPS) was systematically evaluated by three methods pre-adding mode, mixed mode, and post-adding mode. Cellular activity was tested by the CCK-8 assay. The results showed that the optimal modification conditions were the ratio of STMP (%) and STPP (%) 1:4, reaction time 2h and reaction temperature 65°C. Six pRCPS (pRCPS, pRCPS, pRCPS) exhibited significant anti-viral activity in pre-adding mode (P<0.05). Eight pRCPS (pRCPS, pRCPS, pRCPS, pRCPS, and pRCPS showed dramatic anti-viral activity in the mixed mode (P<0.05). Six pRCPS (pRCPS, pRCPS, pRCPS) showed antiviral activity in the post-adding mode (P<0.05). Taken together, four pRCPS (pRCPS) demonstrated significant antiviral activity in all the test modes (P<0.05) and their antiviral efficacy were significantly stronger than unmodified RCPS (P<0.05). Those results indicated that four pRCPS (pRCPS) possessed significant antiviral activity and may have potential as a new CPV therapeutic compound, and phosphorylation could significantly enhance the antiviral effect of RCPS. Moreover, phosphorylation modification technique could be valuable as a method to promote the antiviral activity of polysaccharide. 10.1016/j.ijbiomac.2017.02.085
    [Synthesis of Achyranthes bidentata polysaccharide sulfate and its antivirus activity]. Tian G Y,Li S T,Song M L,Zheng M S,Li W Yao xue xue bao = Acta pharmaceutica Sinica Sulfation of Achyranthes bidentata polysaccharide (Abps) with sulfuric acid or sulfur trioxide-pyridine or chlorosulfonic acid-pyridine was studied. A homogeneous sulfation method with good yield of 82.11% was obtained, using chlorosulfonic acid in an excess of pyridine. Sulfated Achyranthes bidentata polysaccharide was obtained as an amorphous sodium salt easily soluble in water. The UV and IR spectrum of Abps sulfate showed absorptions at 208, 268, 286 nm and 1232, 823.6 cm-1 respectively. The sulfur content of the products was found to be 20-22%. The degree of substitution varied from 2.8 to 3.2. It showed that the hydroxy group of Abps was almost completely esterified by chlorosulfonic acid. The Abps sulfate was shown to have high activity as anti-HBsAg and HBeAg. It is also effective on simple herpes virus type-I.
    Effects of Achyranthes bidentata polysaccharides on interleukin-1 and tumor necrosis factor-alpha production from mouse peritoneal macrophages. Xiang D B,Li X Y Zhongguo yao li xue bao = Acta pharmacologica Sinica Achyranthes bidentata polysaccharides (ABP), extracted from the root of Achyranthes bidentata, induced interleukin-1 (IL-1) synthesis as well as tumor necrosis factor-alpha (TNF-alpha) synthesis and secretion from thioglycolate-primed mouse peritoneal macrophages in vitro. ABP 100-800 micrograms.ml-1 enhanced both synthesis and release of IL-1 when stimulated by lipopolysaccharides (LPS) (5 micrograms.ml-1), but had no significant influences on synthesis and release of TNF-alpha induced by LPS (10 micrograms.ml-1). Studies on IL-1 and TNF-alpha production induced by ABP (200 micrograms.ml-1) alone or plus LPS showed that peak levels of IL-1 release reached at 24 h and that of TNF-alpha release at about 2-6 h after incubation. Peritoneal macrophages from mice ip ABP 25 and 50 mg.kg-1.d-1 x 5 d produced much more IL-1 than those from control group. Peritoneal macrophages from ip ABP 100 mg.kg-1.d-1 x 6 d alone released more TNF-alpha vs control group, and showed a synergetic action with LPS (10 micrograms.ml-1), which was as strong as the positive control agent BCG. These results provide an explanation for the immunopotentiating effect of ABP.
    Antitumor activity and immuno-potentiating actions of Achyranthes bidentata polysaccharides. Xiang D B,Li X Y Zhongguo yao li xue bao = Acta pharmacologica Sinica Achyranthes bidentata polysaccharides (ABP), isolated from the root of Achyranthes bidentata Blume, 50 mg.kg-1 ip or 250 mg.kg-1 to ICR mice inhibited the growth of sarcoma 180. ABP 50 and 100 mg.kg-1 ip prolongated the survival days of mice bearing Ehrlich carcinoma. ABP 50-800 micrograms.ml-1 did not exert direct cytotoxic effect in vitro on S180 cells, but enhanced the cytotoxicity of peritoneal macrophages against S180 cells. ABP 50 mg.kg-1 ip x 17 d or 250 mg.kg-1 ig x 16 d promoted the plaque forming cells (PFC) response to sheep red blood cells (SRBC) and serum IgG level as well as splenocyte proliferation induced by mitogen Con A or LPS in tumor-induced immunodeficient mice. ABP also elevated the NK cell activity and serum TNF content in mice bearing S180. These results indicated that the antitumor effect of ABP may be related to its potentiating effect on both specific and nonspecific host immunological responses.
    Sulfated modification can enhance antiviral activities of Achyranthes bidentata polysaccharide against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. Liu Chuanmin,Chen Huijuan,Chen Kun,Gao Yanfeng,Gao Song,Liu Xiufan,Li Jingui International journal of biological macromolecules Achyranthes bidentata polysaccharide (ABPS) was sulfated using the chlorosulfonic acid-pyridine method. The UV-spectrum of sulfated ABPS (sABPS) was characterized with two different absorbance peaks at 196.3 and 262.8 nm. FT-IR spectrum of sABPS exhibited absorption bands at 1236.5 cm(-1) (asymmetrical S=O stretching vibration) and 822.8 cm(-1) (symmetrical C-O-S vibration related to a C-O-SO(3) group). Anti-PRRSV activities of sABPS were tested on MARC-145 cells by MTT method. The results showed that sABPS exhibited significant antiviral activities at lower concentrations in comparison with the non-modified ABPS treated cells, indicating that sulfated modification could enhance antiviral activities of ABPS. These findings may provide the potential value to develop a new-type drug against PRRSV infection. 10.1016/j.ijbiomac.2012.09.020