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    Melatonin influences on steroidogenic gene expression in the ovary of pinealectomized rats. Maganhin Carla C,Simões Ricardo S,Fuchs Luiz F P,Sasso Gisela R S,Simões Manuel J,Baracat Edmund C,Soares Jose M Fertility and sterility OBJECTIVE:To analyze the expression of genes related to steroidogenesis in the ovary of pinealectomized rats. DESIGN:Experimental research. SETTING:University research laboratory. ANIMAL(S):Thirty female adult rats. INTERVENTION(S):Administration of vehicle (GI), pinealectomy with vehicle (GII), or pinealectomy with melatonin replacement (10 μg/night) for 60 consecutive days (GIII), then euthanasia after 2 months of treatment, ovary collection complementary DNA microarray analyses, confirmatory quantitative reverse-transcriptase polymerase chain reaction analyses, and immunohistochemical analyses for localizing steroidogenesis changes in the ovary. MAIN OUTCOME MEASURE(S):Biologic molecular study followed by immunohistochemical analysis. RESULT(S):The changes in the expression of CYP11A1, CYP17A1, and CYP19A1 after pinealectomy (GII) compared with control (GI) showed the Cyp17a1 expression level increased in the theca interna and interstitial cells in the GII rats compared with the other groups. CONCLUSION(S):Melatonin deprivation (pinealectomy) or administration may influence the ovarian CYP17A1 expression and steroidogenesis. 10.1016/j.fertnstert.2014.04.006
    Evidence that Melatonin Increases Inhibin Beta-A and Follistatin Gene Expression in Ovaries of Pinealectomized Rats. Maganhin Carla C,Baracat Maria Cândida P,Carvalho Kátia C,Seganfredo Isadora Braga,Luquetti Camilla Maganhin,Dos Santos Simões Ricardo,Carbonel Adriana Aparecida Ferraz,de Jesus Simões Manuel,Cipolla-Neto José,Girão Manoel João Batista Castello,Baracat Edmund C,Soares-Jr José M Reproductive sciences (Thousand Oaks, Calif.) Melatonin plays an important role in the regulation of ovarian function including oocyte maturation in different mammalian species. Many studies indicate that melatonin has an impact on the ovarian function of a variety of ovarian cells. However, the information on the exact mechanism and involved hormones is low. To evaluate inhibin beta-A (INHBA) and follistatin (FST) expression in the ovaries of pinealectomized rats treated with melatonin, thirty adult female Wistar rats were randomized into three groups of ten animals each: group 1 (GSh), sham-operated controls receiving vehicle; group 2 (GPx), pinealectomized animals receiving vehicle; and group 3 (GPxMe), pinealectomized animals receiving replacement melatonin (1.0 mg/kg body weight. It was assumed that each animal drank 6.5 ± 1.2 ml per night and weighs approximately 300 g.) for 60 consecutive days. The ovaries were collected for mRNA abundance and protein of INHBA and FST by qRT-PCR and immunohistochemical analyses, respectively. Treatment with melatonin resulted in the upregulation of INHBA and FST genes in the ovarian tissue of the melatonin-treated animals (GPxMe), when compared with GPx. These findings were then confirmed by analyzing the expression of protein by immunohistochemical analyses, which revealed higher immunoreactivity of INHBA and FST in GPxMe animals in the follicular cells compared with GSh and GPx rats. Melatonin increases the expression of INHBA and FST in the ovaries of pinealectomized female rats. 10.1007/s43032-020-00162-1